Energy consumption differences of two cycling garments during short-term cycling were studied. Eleven amateur male cyclists participated in two cycling sessions over two days while wearing a newly designed compression cycling skinsuit (CCS) with stripes simulating kinesio tape, and a conventional compression garment (CG) (control garment). In each session, the participants performed a set of 12 short-term cycling combinations of three workloads and four cadences with either the CCS or the CG. Each combination lasted for 30 s. Garment pressure values at the thigh, oxygen consumption (VO2) and heart rate (HR) were collected and analyzed.